Mouse thromboxane A2 receptor: cDNA cloning, expression and northern blot analysis
T Namba, Y Sugimoto, M Hirata, Y Hayashi… - Biochemical and …, 1992 - Elsevier
T Namba, Y Sugimoto, M Hirata, Y Hayashi, A Honda, A Watabe, M Negishi, A Ichikawa…
Biochemical and biophysical research communications, 1992•ElsevierA cDNA clone for the mouse thromboxane A 2 receptor was isolated from mouse lung cDNA
library. The cDNA has a 1,023 base pair open reading frame which encodes a protein of 341
amino acid residues. STA 2 and U-46619 induced inward current in Xenopus laevis oocytes
injected with the transcript of the clone. Specific binding of [3 H] S-145 was found in
membranes of COS-1 cells transfected with the cDNA (Kd= 3.3 nM) and was displaced with
unlabeled prostaglandins and thromboxane analogues in the order of S-145> STA 2> U …
library. The cDNA has a 1,023 base pair open reading frame which encodes a protein of 341
amino acid residues. STA 2 and U-46619 induced inward current in Xenopus laevis oocytes
injected with the transcript of the clone. Specific binding of [3 H] S-145 was found in
membranes of COS-1 cells transfected with the cDNA (Kd= 3.3 nM) and was displaced with
unlabeled prostaglandins and thromboxane analogues in the order of S-145> STA 2> U …
A cDNA clone for the mouse thromboxane A2 receptor was isolated from mouse lung cDNA library. The cDNA has a 1,023 base pair open reading frame which encodes a protein of 341 amino acid residues. STA2 and U-46619 induced inward current in Xenopus laevis oocytes injected with the transcript of the clone. Specific binding of [3H]S-145 was found in membranes of COS-1 cells transfected with the cDNA (Kd=3.3nM) and was displaced with unlabeled prostaglandins and thromboxane analogues in the order of S-145 > STA2 > U-46619 > PGD2 > PGF2α = PGE2. Northern blot analysis demonstrated that thromboxane A2 receptor mRNA is expressed abundantly in thymus, spleen and lung.
Elsevier
