The Human Immunomodulatory CD25+ B Cell Population belongs to the Memory B Cell Pool
S Amu, A Tarkowski, T Dörner… - Scandinavian …, 2007 - Wiley Online Library
Scandinavian journal of immunology, 2007•Wiley Online Library
We have shown that human CD20+ 25+ B cells display immunomodulatory properties. The
aim of this study was to investigate if CD25+ B cells are found within the CD27 memory B
cell population, and to analyse pattern of their cytokine production. B cells isolated from
healthy subjects, rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients
were analysed regarding the frequency of CD25+ B cells within certain B cell subsets.
Purified CD25+ B cells from healthy subject were used in vitro to evaluate their production of …
aim of this study was to investigate if CD25+ B cells are found within the CD27 memory B
cell population, and to analyse pattern of their cytokine production. B cells isolated from
healthy subjects, rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients
were analysed regarding the frequency of CD25+ B cells within certain B cell subsets.
Purified CD25+ B cells from healthy subject were used in vitro to evaluate their production of …
Abstract
We have shown that human CD20+25+ B cells display immunomodulatory properties. The aim of this study was to investigate if CD25+ B cells are found within the CD27 memory B cell population, and to analyse pattern of their cytokine production.
B cells isolated from healthy subjects, rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) patients were analysed regarding the frequency of CD25+ B cells within certain B cell subsets. Purified CD25+ B cells from healthy subject were used in vitro to evaluate their production of immunomodulatory cytokines.
In healthy subjects the majority (60%) of memory B cells (CD20+27+) also co‐expressed CD25 while only 10–20% of the naïve B cells (CD20+27−) and plasmablasts (CD20–27+) expressed CD25. In RA and SLE patients, we found that 51% and 48%, respectively, co‐expressed CD25 in the memory population, whereas only 11% and 9% co‐expressed CD25 in the naïve B cell population.
Phenotypic analysis of the CD20+25+27+ and CD20+25+27− cells using CD10, CD24, CD38, CD45, CD71, CD80, CD86, CD95, CD138, BAFF‐R, TACI, IgA, IgD, IgG and IgM showed that CD20+25+27+ B cells preferentially represent highly activated, Ig class switched memory B cells.
Cytokine profile analysis showed that CD25+ B cells secreted significantly higher levels of IL‐10 versus CD25− B cells. In contrast, TGF‐β1 secretion was similar between the CD25+ and CD25− sub‐populations.
In conclusion, CD20+25+ B cells constitute a unique subpopulation preferentially occurring among CD20+27+ memory B cells. We suggest that CD25 can be used as a marker for a memory B cell subset.
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