Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid

P Quinn, JF Kerin, GM Warnes - Fertility and sterility, 1985 - Elsevier
Significantly more mouse zygotes developed to blastocysts in culture in a medium
formulated on the composition of human tubal fluid (HTF) than in modified Tyrode's medium
(T6). In a randomized 2× 2 factorial trial of human in vitro fertilization that compared the two
media and culture under oil versus culture in loosely capped tubes, significantly more
clinical pregnancies (30% of 60 transfers) were obtained with HTF medium than with T6
medium (11% of 53 transfers). Decreasing the K+ content of HTF medium to that present in …