Cas-analyzer: an online tool for assessing genome editing results using NGS data
Genome editing with programmable nucleases has been widely adopted in research and
medicine. Next generation sequencing (NGS) platforms are now widely used for measuring
the frequencies of mutations induced by CRISPR-Cas9 and other programmable nucleases.
Here, we present an online tool, Cas-Analyzer, a JavaScript-based implementation for NGS
data analysis. Because Cas-Analyzer is completely used at a client-side web browser on-the-
fly, there is no need to upload very large NGS datasets to a server, a time-consuming step in …
medicine. Next generation sequencing (NGS) platforms are now widely used for measuring
the frequencies of mutations induced by CRISPR-Cas9 and other programmable nucleases.
Here, we present an online tool, Cas-Analyzer, a JavaScript-based implementation for NGS
data analysis. Because Cas-Analyzer is completely used at a client-side web browser on-the-
fly, there is no need to upload very large NGS datasets to a server, a time-consuming step in …
Summary
Genome editing with programmable nucleases has been widely adopted in research and medicine. Next generation sequencing (NGS) platforms are now widely used for measuring the frequencies of mutations induced by CRISPR-Cas9 and other programmable nucleases. Here, we present an online tool, Cas-Analyzer, a JavaScript-based implementation for NGS data analysis. Because Cas-Analyzer is completely used at a client-side web browser on-the-fly, there is no need to upload very large NGS datasets to a server, a time-consuming step in genome editing analysis. Currently, Cas-Analyzer supports various programmable nucleases, including single nucleases and paired nucleases.
Availability and Implementation
Free access at http://www.rgenome.net/cas-analyzer/.
Supplementary information
Supplementary data are available at Bioinformatics online.
Oxford University Press