The extracellular matrix (ECM) is recognized as a diverse, dynamic, and complex
environment that is involved in multiple cell-physiological and pathological processes.
However, the isolation of ECM, from tissues or cell culture, is complicated by the insoluble
and cross-linked nature of the assembled ECM and by the potential contamination of ECM
extracts with cell surface and intracellular proteins. Here, we describe a method for use with
cultured cells that is rapid and reliably removes cells to isolate a cell-derived ECM for …