Fluorescence assay for studying the ability of macrolides to induce production of ribosomal methylase
G Clarebout, R Leclercq - Antimicrobial agents and chemotherapy, 2002 - Am Soc Microbiol
G Clarebout, R Leclercq
Antimicrobial agents and chemotherapy, 2002•Am Soc MicrobiolABSTRACT A screening assay to test the inducing capacity of macrolides by fusing the
attenuator of the inducible erm (B) gene from Streptococcus pneumoniae HM28 with the
gfpmut1 gene has been designed. Fluorescence was detected under UV light around disks
impregnated with inducer macrolides (erythromycin or azithromycin) but not with noninducer
ketolides. Induction could be quantified by fluorometry.
attenuator of the inducible erm (B) gene from Streptococcus pneumoniae HM28 with the
gfpmut1 gene has been designed. Fluorescence was detected under UV light around disks
impregnated with inducer macrolides (erythromycin or azithromycin) but not with noninducer
ketolides. Induction could be quantified by fluorometry.
Abstract
A screening assay to test the inducing capacity of macrolides by fusing the attenuator of the inducible erm(B) gene from Streptococcus pneumoniae HM28 with the gfpmut1 gene has been designed. Fluorescence was detected under UV light around disks impregnated with inducer macrolides (erythromycin or azithromycin) but not with noninducer ketolides. Induction could be quantified by fluorometry.
American Society for Microbiology