Absolute quantification by droplet digital PCR versus analog real-time PCR
CM Hindson, JR Chevillet, HA Briggs, EN Gallichotte… - Nature …, 2013 - nature.com
CM Hindson, JR Chevillet, HA Briggs, EN Gallichotte, IK Ruf, BJ Hindson, RL Vessella…
Nature methods, 2013•nature.comNanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly
precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by
ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased 37–
86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with
comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis,
this translated to superior diagnostic performance for identifying individuals with cancer.
precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by
ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased 37–
86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with
comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis,
this translated to superior diagnostic performance for identifying individuals with cancer.
Abstract
Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of variation decreased 37–86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis, this translated to superior diagnostic performance for identifying individuals with cancer.
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