Protein kinase Cϵ activates protein kinase B/Akt via DNA-PK to protect against tumor necrosis factor-α-induced cell death
D Lu, J Huang, A Basu - Journal of Biological Chemistry, 2006 - ASBMB
We have previously shown that protein kinase Cϵ (PKCϵ) protects breast cancer cells from
tumor necrosis factor-α (TNF)-induced cell death. In the present study, we have investigated
if the antiapoptotic function of PKCϵ is mediated via Akt and the mechanism by which PKCϵ
regulates Akt activity. TNF caused a transient increase in Akt phosphorylation at Ser 473 in
MCF-7 cells. Overexpression of PKCϵ in MCF-7 cells increased TNF-induced Akt
phosphorylation at Ser 473 resulting in its activation. Knockdown of PKCϵ by small …
tumor necrosis factor-α (TNF)-induced cell death. In the present study, we have investigated
if the antiapoptotic function of PKCϵ is mediated via Akt and the mechanism by which PKCϵ
regulates Akt activity. TNF caused a transient increase in Akt phosphorylation at Ser 473 in
MCF-7 cells. Overexpression of PKCϵ in MCF-7 cells increased TNF-induced Akt
phosphorylation at Ser 473 resulting in its activation. Knockdown of PKCϵ by small …
