Detection of conserved N-linked glycans and phase-variable lipooligosaccharides and capsules from campylobacter cells by mass spectrometry and high resolution …
CM Szymanski, FS Michael, HC Jarrell, J Li… - Journal of Biological …, 2003 - ASBMB
Glycomics, the study of microbial polysaccharides and genes responsible for their formation,
requires the continuous development of rapid and sensitive methods for the identification of
glycan structures. In this study, methods for the direct analysis of sugars from 10 8 to 10 10
cells are outlined using the human gastrointestinal pathogen, Campylobacter jejuni. Using
capillary-electrophoresis coupled with sensitive electrospray mass spectrometry, we
demonstrate variability in the lipid A component of C. jejuni lipooligosaccharides (LOSs). In …
requires the continuous development of rapid and sensitive methods for the identification of
glycan structures. In this study, methods for the direct analysis of sugars from 10 8 to 10 10
cells are outlined using the human gastrointestinal pathogen, Campylobacter jejuni. Using
capillary-electrophoresis coupled with sensitive electrospray mass spectrometry, we
demonstrate variability in the lipid A component of C. jejuni lipooligosaccharides (LOSs). In …