Targeting p53 as a general tumor antigen.
M Theobald, J Biggs, D Dittmer… - Proceedings of the …, 1995 - National Acad Sciences
M Theobald, J Biggs, D Dittmer, AJ Levine, LA Sherman
Proceedings of the National Academy of Sciences, 1995•National Acad SciencesA major barrier to the design of immunotherapeutics and vaccines for cancer is the
idiosyncratic nature of many tumor antigens and the possibility that T cells may be tolerant of
broadly distributed antigens. We have devised an experimental strategy that exploits
species differences in protein sequences to circumvent tolerance of high-affinity T cells. HLA
transgenic mice were used to obtain cytotoxic T lymphocytes specific for peptides from the
human p53 tumor-suppressor molecule presented in association with HLA-A2. 1. Although …
idiosyncratic nature of many tumor antigens and the possibility that T cells may be tolerant of
broadly distributed antigens. We have devised an experimental strategy that exploits
species differences in protein sequences to circumvent tolerance of high-affinity T cells. HLA
transgenic mice were used to obtain cytotoxic T lymphocytes specific for peptides from the
human p53 tumor-suppressor molecule presented in association with HLA-A2. 1. Although …
A major barrier to the design of immunotherapeutics and vaccines for cancer is the idiosyncratic nature of many tumor antigens and the possibility that T cells may be tolerant of broadly distributed antigens. We have devised an experimental strategy that exploits species differences in protein sequences to circumvent tolerance of high-affinity T cells. HLA transgenic mice were used to obtain cytotoxic T lymphocytes specific for peptides from the human p53 tumor-suppressor molecule presented in association with HLA-A2.1. Although such p53-specific cytotoxic T cells did not recognize nontransformed human cells, they were able to lyse a wide variety of human tumor cells lines, thus confirming the existence of broadly distributed determinants that may serve as targets for immunotherapy.
National Acad Sciences